<p>We describe methclone, a novel method to identify epigenetic loci that harbor large changes in the clonality of their epialleles (epigenetic alleles). Methclone efficiently analyzes genome-wide DNA methylation sequencing data. We quantify the changes using a composition entropy difference calculation and also introduce a new measure of global clonality shift, loci with epiallele shift per million loci covered, which enables comparisons between different samples to gauge overall epiallelic dynamics. Finally, we demonstrate the utility of methclone in capturing functional epiallele shifts in leukemia patients from diagnosis to relapse. Methclone is open-source and freely available at <a href="https://code.google.com/p/methclone">https://code.google.com/p/methclone</a>. Reprinted with permission from BMC Publishing (Genome Biology).</p> <p> <img src="GetImage.cgi?study_id=phs000793&#x26;image_name=Methclone.jpg"> </p>

Paired AML Epigenetics Study on Clonality

An optimized algorithm for detecting and annotating regional differential methylation

Base-Pair Resolution DNA Methylation Sequencing Reveals Profoundly Divergent Epigenetic Landscapes in Acute Myeloid Leukemia

Deficiency of the murine fifth complement component (C5). A 2-base pair gene deletion in a 5'-exon.