<p>GABAergic interneurons are essential for neural circuit function and their loss or dysfunction is implicated in human neuropsychiatric disease. In vitro methods for interneuron generation hold promise for studying human cellular and functional properties and ultimately therapeutic cell replacement. We used a protocol for generating cortical interneurons from hESCs and analyzed the properties and maturation timecourse of cell types using single-cell RNAseq (data available at <a href="https://www.ncbi.nlm.nih.gov/geo/">GEO</a> under: GSE93802 on March 10 2017). Transcriptomic profiles of the hESC-derived interneurons were compared to several different populations of cells from mid-gestation human neocortex that showed differing levels of <a href="https://www.ncbi.nlm.nih.gov/gene/5080">PAX6</a> and <a href="https://www.ncbi.nlm.nih.gov/gene/6657">SOX2</a> expression.</p> <p>For this study, 104 samples of 100 human neocortical cells each, have been sorted based on <a href="https://www.ncbi.nlm.nih.gov/gene/6657">SOX2</a> and <a href="https://www.ncbi.nlm.nih.gov/gene/5080">PAX6</a> expression, mRNA recovered from the fixed cells by FRISCR, and transcriptomic profiles generated by SmartSeq2. The RNA-seq data from the 104 100-cell samples is included in this dbGaP study.</p>

Single-Cell Profiling of hESC-Derived Interneuron Development

2 selected publications including "Fixed single-cell transcriptomic characterization of human radial glia..." (222 citations)

Single-Cell Profiling of hESC-Derived Interneuron Development

Fixed single-cell transcriptomic characterization of human radial glial diversity

A Single-Cell Roadmap of Lineage Bifurcation in Human ESC Models of Embryonic Brain Development.