<p>In this study, we analyzed a series of colorectal cancers using a new sequencing technology that sequences more than 200 microsatellite regions in parallel. With this technology, we evaluated mono-, di- , tri- and tetranucleotide repeats to provide a broad spectrum of microsatellite instability (MSI) events. This deep sequencing technology provides greater than 2000X coverage and eliminates the need for PCR amplification. Because every read corresponds to a single DNA molecule, we eliminated artifacts introduced by PCR that obscured true MSI events. Also included in our analysis is a new method for detection of somatic copy number changes in 83 cancer genes, with which we can infer chromosomal instability (CIN) status.</p>

Improved Detection and Identification of Microsatellite Instability    Features in Colorectal Cancer

17 variables across Demographics, Disease Events, Race and Ethnicity

Improved Detection and Identification of Microsatellite Instability Features in Colorectal Cancer

Demographics (1)

Disease Events (5)

Race and Ethnicity (1)

Other (10)