<p><b><u>Pneumococcal Vaccine Study</u>:<u> </u></b></p><div>This is a study designed to develop a detailed transcriptional and cellular profile of the immune response to pneumococcal vaccination with conjugated and non-conjugated polysaccharide vaccines in the senescent immune system of older adults. </div><br><div>In this study, 39 healthy adults ages 60 and older that have never received pneumococcal vaccination, have been randomized to receive Prevnar 13 (Pfizer), a conjugated 13-valent vaccine or Pneumovax 23 (Merck), a non-conjugated 23-valent vaccine. Four (4) study visits occurred over 70 days at Day -7 (baseline), day 1, day 10 and day 60 (± 5d) post vaccination.Bulk RNA-sequencing data are available on the 4 time points for 31 of the participants and 1 replicate.</div><br><div> </div><div><b><u>ATAC-Doublet Detector Study</u>:<u> </u></b></div><div>We generated single nucleus ATAC-seq (snATAC-seq) data from PBMCs of 2 baseline blood samples from the pneumococcal study. Generated data was then used to benchmark a novel computational method for detecting multiplets in snATAC-seq data, namely, AMULET (<a href="https://github.com/UcarLab/AMULET">https://github.com/UcarLab/AMULET</a>). Multiplets confound downstream analyses by introducing combined epigenomic profiles from 2 or more cells/nuclei. AMULET detects multiplets by using unique snATAC-seq data features that allow it to reliably use read counts to detect multiplets for their removal from data analyses. </div><br><div> </div><p></p>

Genomics and Epigenomics of the Elderly Response to Pneumococcal Vaccines

AMULET: a novel read count-based method for effective multiplet detection from single nucleus ATAC-seq data

Distinct baseline immune characteristics associated with responses to conjugated and unconjugated pneumococcal polysaccharide vaccines in older adults