<p>We developed a high-dimensional, tetramer associated T cell receptor sequencing (TetTCR-SeqHD) method to simultaneously profile cognate antigen specificities, TCR sequences, targeted gene-expression, and surface protein expression. Cultured antigen-specific CD8+ T cell clones were studied as a proof-of-concept experiment. In addition, both the antigen specific CD8+ T cells from healthy subjects and Type I diabetic patients were investigated. </p><ul><li>18 individually consented subjects pooled into four groups for sample processing<br></li></ul>

High-Throughput and High-Dimensional Single Cell Analysis of    Antigen-Specific CD8+ T Cells

Diabetes Mellitus, Type 1 study with AMPLICON data on dbGaP (22 participants)