<p>To explore tumor heterogeneity in sporadic human basal cell carcinoma tumors, we profiled three individual patient tumors by scRNA-Seq and scATAC-Seq. Basal cell carcinomas (BCCs) are driven by hedgehog signaling with <a href="https://www.ncbi.nlm.nih.gov/gene/2735">GLI1</a> representing the key downstream transcription factor that drives tumor growth. <a href="https://www.ncbi.nlm.nih.gov/gene/2735">GLI1</a> as such is a readout of active hedgehog signaling. We identified that <a href="https://www.ncbi.nlm.nih.gov/gene/8581">LY6D</a> can mark squamous populations such as in squamous cell carcinomas (SCCs) that are negative for <a href="https://www.ncbi.nlm.nih.gov/gene/2735">GIL1</a> and hedgehog signaling. As such, both <a href="https://www.ncbi.nlm.nih.gov/gene/2735">GLI1</a> and <a href="https://www.ncbi.nlm.nih.gov/gene/8581">LY6D</a> can be used as biomarkers of BCCs and SCCs and their related states.</p>

Single Cell Analysis of Sporadic Human Basal Cell Carcinomas

LY6D marks pre-existing resistant basosquamous tumor subpopulations