<p>This study examined the transcriptional changes induced by cancer associated fibroblasts (CAFs) in pancreatic ductal adenocarcinoma patient-derived tumor organoids. We hypothesized that cancer associated fibroblasts impacted tumor cell transcription to promote tumorigenic behavior. Using patient-matched cells from 3 patients, we compared the transcriptional profiles of patient-derived organoids in monoculture to patient-derived organoids co-cultured with CAFs. Similarly, we looked at the transcriptional changes in matched patient-derived cancer associated fibroblasts grown in monoculture or co-cultured with patient-derived organoids. With a focus on intercellular crosstalk, we identified crosstalk between cancer associated fibroblasts and patient-derived organoids through <a href="https://www.ncbi.nlm.nih.gov/gene/7422">VEGFA</a> and <a href="https://www.ncbi.nlm.nih.gov/gene/8829">NRP1</a>. This was identified by bulk RNA sequencing and examined dynamically in organoid-CAF co-culture. This dbGaP deposit is the bulk RNA sequencing data for these 3 patients. </p>

Transfer Learning Associates CAFs with EMT and Inflammation in Tumor    Cells in Human Tumors and Organoid Co-Culture in Pancreatic Ductal    Adenocarcinoma

1 selected publication including "Transfer learning reveals cancer-associated fibroblasts are associated..." (23 citations)

Selected Publications — phs003563 — Transfer Learning Associates CAFs with EMT and Inflammation in Tumor    Cells in Human Tumors and Organoid Co-Culture in Pancreatic Ductal    Adenocarcinoma

Transfer Learning Associates CAFs with EMT and Inflammation in Tumor Cells in Human Tumors and Organoid Co-Culture in Pancreatic Ductal Adenocarcinoma

Transfer learning reveals cancer-associated fibroblasts are associated with epithelial-mesenchymal transition and inflammation in cancer cells in pancreatic ductal adenocarcinoma.