Using a patient derived model of small cell lung cancer, the <a href="https://www.ncbi.nlm.nih.gov/gene/9817">KEAP1</a> gene was deleted using lentiviral expression of a vector expressing CAS9 and single guide RNA (sgRNA) targeting <a href="https://www.ncbi.nlm.nih.gov/gene/9817">KEAP1</a>. A control vector expressing model was also generated. RNA-Seq analysis was performed to compare gene expression in tumors growing in vivo, comparing <a href="https://www.ncbi.nlm.nih.gov/gene/9817">KEAP1</a> deleted to control tumors under saline-treated and cisplatin/etoposide-treated conditions.

Identifying and Understanding Drivers of Chemoresistance in Small Cell    Lung Cancer

1 selected publication including "In vivo functional screens reveal KEAP1 loss as a driver of chemoresis..." (2 citations)

Identifying and Understanding Drivers of Chemoresistance in Small Cell Lung Cancer

In vivo functional screens reveal KEAP1 loss as a driver of chemoresistance in small cell lung cancer